Josefina Eugenia Vazquez Mota, Mexican politician and presidential candidate
Josefina Pla, Spanish poet
Josefina de Vasconcellos, Brazilian-British sculptor
(María) Josefina Sruoga, Argentine Olympic field hockey player
Josefina Maria Niggli, Mexican-American playwright and novelist
Josefina López, American playwright
Josefina Molina, Spanish film director
Josefina Montoya, character in the American Girl doll collection
Josefina LaCosta, character in the Richard Sharpe series of novels by Bernard Cornwell.
Josefina, GilHarris' fake girlfriend in movie "The NewGuy"
Josefina Menendez, character in video game "Call of Duty: Black Ops II"